Composite

Part:BBa_K1861020:Design

Designed by: Vladyslav Vyshnevskyi   Group: iGEM15_FAU_Erlangen   (2015-09-18)

Fusion protein of the TALE constant domain and a SpyTag in an expression cassette


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1256
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 260
    Illegal XhoI site found at 1052
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 707
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used the BamHI and SacI restriction sites to clone the TALE constant domain into the expression cassette BBa_K1861010.
Both the cassette BBa_K1861010 and the constant domain with the SpyTag BBa_K1861201 have been ordered from IDT.

Source

The rpd3 gene is located on chromosome XIV of S. cerevisiae. The promoter sequence can be found in the Yeast Promoter Atlas.
The sequence of the TALE constant domain was obtained using the TAL Plasmids Sequence Assembly Tool.
For the SpyTag and the terminator, we used the sequences of BBa_K1159201 and BBa_K392003, respectively.

References

https://eu.idtdna.com/site, visited on 2015-09-19
http://ypa.csbb.ntu.edu.tw/do?act=gene_by_kw&query=rpd3, visited on 2015-09-19
http://bao.rice.edu/Research/BioinformaticTools/assembleTALSequences.html, visited on 2015-09-20
http://web.archive.org/web/20150920175258/http://bao.rice.edu/Research/BioinformaticTools/assembleTALSequences.html