Device

Part:BBa_K185034:Design

Designed by: Shuai Wang   Group: iGEM09_SJTU-BioX-Shanghai   (2009-10-16)

Promoter J23116+Q04510+B0034+RelB+Double-terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1159
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is based on QPI(Q04510), which made by early team. We add a promoter(J23116) on the begining of QPI and add RBS(B0030), RelB(K185048) gene and Double-terminator(B0015) at the end of it in order to express RelB protein. Because of QPI contain CI gene so that this device can be induced by Tryptophan.


Source

None

References