Coding

Part:BBa_K1850013:Design

Designed by: Lydia Goldberg   Group: iGEM15_Harvard_BioDesign   (2015-09-15)

pAra - fim


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 2715
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 998
    Illegal AgeI site found at 1029
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We selected BBa_I13453, an arabinose promoter, because it is a titratable promoter that allows for controlled expression of pili structural genes over a broad range of inducer concentrations.

fim was amplified with the native ribosomal binding sites, so that we could maintain the dosing of the fim subunits.


Source

The type I pili structural and transport genes from the fim operon were amplified from the E. coli K-12 genome.

References