Coding

Part:BBa_K1845003:Design

Designed by: Edoardo Gianni   Group: iGEM15_London_Biohackspace   (2015-09-18)


ATF1 - Fruity CDS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1592
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1592
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 99
    Illegal BamHI site found at 1426
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1592
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1592
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 526
    Illegal SapI.rc site found at 1534


Design Notes

Synthesised as gBlock, codon optimised and his-tagged on genome compiler,


Source

Sequence from NCBI Saccharomyces carlsbergensis (D63449)

References