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Part:BBa_K1844004:Design

Designed by: David Lips   Group: iGEM15_Amsterdam   (2015-09-17)


Arg-H KO Construct


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 959
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 959
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 959
    Illegal NgoMIV site found at 257
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Removed one illegal restriction site via site-directed mutagenesis.


Source

It contains the homologous upstream and downstream regions of the arg-H gene from the Synechocystis genome.

References