Coding

Part:BBa_K1842001:Design

Designed by: Walter Thavarajah   Group: iGEM15_UC_San_Diego   (2015-08-17)


LuxCDEC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 601
    Illegal BglII site found at 2813
    Illegal BglII site found at 4369
    Illegal BamHI site found at 3570
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3247
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 90
    Illegal BsaI site found at 3858


Design Notes

The coding sequence was too large to by synthesized in one piece, so it was instead synthesized in a series of fragments that were assembled via Gibson Assembly.


Source

Each of the genes used was derived from the marine bioluminescent bacterium Photobacterium Phosphoreum.

References