Generator

Part:BBa_K1833008:Design

Designed by: Konstantin Borisov   Group: iGEM15_Pitt   (2015-09-17)

pT7-MMP9 sensitive repressor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 64
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 297
    Illegal NgoMIV site found at 696
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 194
    Illegal SapI.rc site found at 53

References

G. Di Lallo, L. Castagnoli, P. Ghelardini and L. Paolozzi. A two-hybrid system based on chimeric operator recognition for studying protein homo/heterodimerization in Escherichia coli. Microbiology (2001), 147, 1651–1656