Part:BBa_K1829002:Design

pFig2c: Mating factor alpha responsive promoter

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 431
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 125
  Illegal BsaI.rc site found at 564
  

Design Notes

We changed nucleeotide 22 from adenine to thymine in order to eliminate the illegal Pst1 cut site.

Source

pFig2c is found in the genomic sequence of MATa S. cerevisiae.