Part:BBa_K1824008

Consensus Promoter T7 + RNA Thermometer U6

This is a composite regulatory part that consisting of T7 promoter BBa_I13453 and RNA Thermometer U6 BBa_K1824564 for temperature induced gene expression at 37 degrees. The possible secondary structure of A1 was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.

This is a composite regulatory part that consisting of T7 promoter BBa_I13453 and RNA Thermometer U6 BBa_K1824564 for temperature induced gene expression at 37 degrees. The possible secondary structure of U6 was simulated by RNAstructure (Fig.1). This combination is compatible with the assembly method that XJTLU-CHINA promoted.

Figure 1: Possible secondary structure of RNAT U6.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal SpeI site found at 155
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 125
  Illegal SpeI site found at 155
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 65
  Illegal BamHI site found at 141
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal SpeI site found at 155
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal SpeI site found at 155
  
• 1000
  COMPATIBLE WITH RFC[1000]