Composite

Part:BBa_K1824005:Design

Designed by: Wenbo Xu   Group: iGEM15_XJTLU-CHINA   (2015-09-06)


pBAD + RNA Thermometer U6


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 153
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal SpeI site found at 153
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal BamHI site found at 139
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 153
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 153
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The transcription of promoters always came with redundancy, which means the last few nucleic acids of the promoter can also be transcribed. It is important to avoid truncation or alteration of the RNA sequence


Source

U6 was from registry.

References