Composite
Part:BBa_K1824000:Design
Designed by: Wenbo Xu, Hao Wu Group: iGEM15_XJTLU-CHINA (2015-09-04)
Constitutive Promoter J23119 + RNA Thermometer A1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The transcription of promoters always came with redundancy, which means the last few nucleic acids of the promoter can also be transcribed. It is important to avoid truncation or alteration of the RNA sequence.
Source
J23119 was from the Registry, and A1 was from BIT-CHINA.