Protein_Domain

Part:BBa_K1821000:Design

Designed by: Dr. Ankush Garg, Kevin Klemmer   Group: iGEM15_Central_Calgary   (2015-09-17)

Angiostatin is an antiangiogenic protein.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Angiostatin is a sequence taken from a larger protein sequence but will function outside of the larger protein.


Source

The DNA for our protein was modified to only use the first three Kringles of the human plasminogen. because the most effective inhibition of endothelial cell growth occurs in these Kringles. It was also modified it to prevent glycosylation so that the protein would not be destroyed when secreted into a mammal.

References

Kringle Domains of Human Angiostatin Charaterization of the Anti-Proliferative Activity of Endothelial Cells http://www.jbc.org/content/271/46/29461


The entire angiostatin protein is from amino acid 79-466: http://www.uniprot.org/uniprot/P00747#PRO_0000028057

Entire DNA Sequence: http://www.ncbi.nlm.nih.gov/nuccore/NC_000006.12?report=fasta&from=160702193&to=160754054