Signalling
Part:BBa_K1819002:Design
Designed by: Lais Ribovski Group: iGEM15_Brasil-USP (2015-09-13)
Twin-arginine translocation signal (TAT signal)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
SacI restriction site was added at the 3’ flanking region of TAT signal to link it at the first amino acid residue of the protein to be exported.
Source
Hydrogenase-1 small chain precursor from Escherichia coli