Composite

Part:BBa_K1813062

Designed by: UBC iGEM 2015   Group: iGEM15_British_Columbia   (2015-09-11)

ptac pelB AgCPR pelb CYP6CM1

With ptac powering the transcription of BBa_K1813053 and BBa_K1813051, this part allows the co-localization of large amounts of simultaneously expressed CYP6CM1 ( BBa_K1813003) and A. gambiae NADPH p450 reductase ( BBa_K1813010) to the bacterial periplasm.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 626
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 141
    Illegal NgoMIV site found at 2448
    Illegal AgeI site found at 424
    Illegal AgeI site found at 1606
    Illegal AgeI site found at 1762
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2122


[edit]
Categories
Parameters
None