Coding

Part:BBa_K1809021:Design

Designed by: David Morgan   Group: iGEM15_WPI-Worcester   (2015-08-26)


ZeAFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 16
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 205
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized the sequence for E. coli and to minimize repetitive sequences; removed illegal restriction sites.


Source

Isolated from the part BBa_K652004 taken from the 2015 distribution plate 1, well 10C.

References