Part:BBa_K1806006:Experience

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Cloning

The sponsors providing the parts were not able to synthesize orders of more than 2000 bp. For this reason, the part was split into two respective segment parts to be ligated. The two segments were tagged as G-Block 10 and 11. Initially the G-Blocks were ligated to the pSB1C3 vector to be cloned. The verified cloning of the segment parts can be seen in the gel images below.

The two segment parts were ligated from the Hind3 restriction site. The ligated 10+11 urease complete part was cut with the EcoR1 and Pst1 restriction enzymes. The bands had the appropriate base lengths in the gel runs.

User Reviews

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