Composite

Part:BBa_K180019:Design

Designed by: Richard Wilson, Stephanie Chambers   Group: iGEM09_Southampton   (2009-10-19)


Scissors - Primary plasmid (part 2) [tac pR, mBanana and LuxI generator]


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1825
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1825
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1825
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1825
    Illegal NgoMIV site found at 2071
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2014


Design Notes

Regulated expression of LasI (C0178) and mBanana (K180009) by tac pR (K180000), controlled by presence of lac/IPTG or trp in the cell.


Source

See relevant basic biobrick parts (K180000, B0034, K180009, B0015).

References