Coding

Part:BBa_K1800002:Design

Designed by: Laura Lee Irvin   Group: iGEM15_Georgia_State   (2015-08-28)

Horseradish Peroxidase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 154
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 396
    Illegal XhoI site found at 480
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

HRP was designed to include the RFC 10 prefix and suffix. The start and stop codons were not removed. It is intended to be put into pGAP and pORE vectors for expression in Pichia Pastoris and Agrobacterium.


Source

synthesized by IDT

References