Reporter

Part:BBa_K1797010

Designed by: iGEM15_Tsinghua   Group: iGEM15_Tsinghua   (2015-09-17)

Kana cassette flanked by loxP site

This part includes a translational kanamycin resistance gene(in prokaryotes) or a neomycin resistance gene(in eukaryotes) and flanking loxP sites, which can be recognized by Cre recombinase. We can use DNA recombination technology to knock this part into E.coli genome and the antibiotic resistance gene helps us find the right colonies. Then if Cre is expressed in the bacteria, this kana cassette can be excised and a knock out experiment is completed.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 367
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1031
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 880
    Illegal SapI.rc site found at 1090


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