Coding

Part:BBa_K1796106:Design

Designed by: Tang Yulong   Group: iGEM15_SCU_China   (2015-09-17)


methylene-H4folate reductase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 808
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 808
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 808
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 808
    Illegal NgoMIV site found at 544
    Illegal NgoMIV site found at 652
    Illegal NgoMIV site found at 829
    Illegal NgoMIV site found at 889
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon preference has been changed to suit E.coli. And,some restriction sites have been avoid.


Source

This parts is cloned from the genomic sequence of Moorella thermoacetica

References