Part:BBa_K1796106

methylene-H4folate reductase

This reductase has catalysis of turning methylene-H4folate into methyl-H4folate, then the methyl can be taken by CFeSP.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 808
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 808
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 808
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 808
  Illegal NgoMIV site found at 544
  Illegal NgoMIV site found at 652
  Illegal NgoMIV site found at 829
  Illegal NgoMIV site found at 889
  
• 1000
  COMPATIBLE WITH RFC[1000]

Parameter of Protein

Number of amino acids: 306

Molecular weight: 33924.5

Theoretical pI: 8.27

Amino acid composition: 

Ala (A) 32 10.5%

Arg (R) 18 5.9%

Asn (N) 9 2.9%

Asp (D) 17 5.6%

Cys (C) 4 1.3%

Gln (Q) 15 4.9%

Glu (E) 21 6.9%

Gly (G) 21 6.9%

His (H) 9 2.9%

Ile (I) 26 8.5%

Leu (L) 22 7.2%

Lys (K) 22 7.2%

Met (M) 14 4.6%

Phe (F) 12 3.9%

Pro (P) 14 4.6%

Ser (S) 14 4.6%

Thr (T) 7 2.3%

Trp (W) 1 0.3%

Tyr (Y) 4 1.3%

Val (V) 24 7.8%

Pyl (O) 0 0.0%

Sec (U) 0 0.0%

(B) 0 0.0%

(Z) 0 0.0%

(X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 38

Total number of positively charged residues (Arg + Lys): 40

Atomic composition:Carbon C 1501

Hydrogen H 2435

Nitrogen N 425

Oxygen O 432

Sulfur S 18

Formula: C1501H2435N425O432S18Total number of atoms: 4811

Extinction coefficients:Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 11710

Abs 0.1% (=1 g/l) 0.345, assuming all pairs of Cys residues form cystines

Ext. coefficient 11460

Abs 0.1% (=1 g/l) 0.338, assuming all Cys residues are reduced

Estimated half-life:The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).

>20 hours (yeast, in vivo).

>10 hours (Escherichia coli, in vivo).

Instability index:The instability index (II) is computed to be 36.95

This classifies the protein as stable.

Aliphatic index: 94.38

Grand average of hydropathicity (GRAVY): -0.119