Part:BBa_K1796105
ACS chaperone
This sequence code a portion, which is helping ACS to fold in right way. If you need to product ACS and want it to have functions, this protein is necessary.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 299
Illegal NgoMIV site found at 403
Illegal NgoMIV site found at 422
Illegal NgoMIV site found at 493
Illegal NgoMIV site found at 690 - 1000COMPATIBLE WITH RFC[1000]
Parameter of Protein
Number of amino acids: 446
Molecular weight: 48153.2
Theoretical pI: 5.38
Amino acid composition:
Ala (A) 65 14.6%
Arg (R) 19 4.3%
Asn (N) 19 4.3%
Asp (D) 26 5.8%
Cys (C) 5 1.1%
Gln (Q) 10 2.2%
Glu (E) 31 7.0%
Gly (G) 25 5.6%
His (H) 7 1.6%
Ile (I) 24 5.4%
Leu (L) 49 11.0%
Lys (K) 28 6.3%
Met (M) 6 1.3%
Phe (F) 11 2.5%
Pro (P) 21 4.7%
Ser (S) 21 4.7%
Thr (T) 24 5.4%
Trp (W) 2 0.4%
Tyr (Y) 16 3.6%
Val (V) 37 8.3%
Pyl (O) 0 0.0%
Sec (U) 0 0.0%
(B) 0 0.0%
(Z) 0 0.0%
(X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 57
Total number of positively charged residues (Arg + Lys): 47
Atomic composition:Carbon C 2151
Hydrogen H 3454
Nitrogen N 576
Oxygen O 651
Sulfur S 11
Formula: C2151H3454N576O651S11Total number of atoms: 6843
Extinction coefficients:Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 35090
Abs 0.1% (=1 g/l) 0.729, assuming all pairs of Cys residues form cystines
Ext. coefficient 34840
Abs 0.1% (=1 g/l) 0.724, assuming all Cys residues are reduced
Estimated half-life:The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:The instability index (II) is computed to be 25.50
This classifies the protein as stable.
Aliphatic index: 102.47
Grand average of hydropathicity (GRAVY): 0.008
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