Part:BBa_K1796102
A chain of CODH/ACS beta subunit
The beta subunit of CODH/ACS have four chains, named A,B,C,D. This four chains have same sequence. CODH/ACS has oxidoreductase function， but this function depend on the intact structure,acluding two subunits.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2042 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal SpeI site found at 2043
Illegal PstI site found at 2057
Illegal NotI site found at 7
Illegal NotI site found at 2050 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2043 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 2043
Illegal PstI site found at 2057
Illegal AgeI site found at 341
Illegal AgeI site found at 746
Illegal AgeI site found at 971
Illegal AgeI site found at 1046
Illegal AgeI site found at 1511 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1311
Parameter of Protein
Number of amino acids: 631
Molecular weight: 71751.2
Theoretical pI: 10.03
Amino acid composition:
Ala (A) 33 5.2%
Arg (R) 96 15.2%
Asn (N) 12 1.9%
Asp (D) 18 2.9%
Cys (C) 49 7.8%
Gln (Q) 22 3.5%
Glu (E) 14 2.2%
Gly (G) 60 9.5%
His (H) 28 4.4%
Ile (I) 8 1.3%
Leu (L) 48 7.6%
Lys (K) 7 1.1%
Met (M) 1 0.2%
Phe (F) 15 2.4%
Pro (P) 59 9.4%
Ser (S) 74 11.7%
Thr (T) 16 2.5%
Trp (W) 12 1.9%
Tyr (Y) 40 6.3%
Val (V) 19 3.0%
Pyl (O) 0 0.0%
Sec (U) 0 0.0%
(B) 0 0.0%
(Z) 0 0.0%
(X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 103
Atomic composition:Carbon C 3096
Hydrogen H 4766
Nitrogen N 1028
Oxygen O 860
Sulfur S 50
Formula: C3096H4766N1028O860S50Total number of atoms: 9800
Extinction coefficients:Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 128600
Abs 0.1% (=1 g/l) 1.792, assuming all pairs of Cys residues form cystines
Ext. coefficient 125600
Abs 0.1% (=1 g/l) 1.750, assuming all Cys residues are reduced
Estimated half-life:The N-terminal of the sequence considered is E (Glu).
The estimated half-life is: 1 hours (mammalian reticulocytes, in vitro).
30 min (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:The instability index (II) is computed to be 65.39
This classifies the protein as unstable.
Aliphatic index: 48.57
Grand average of hydropathicity (GRAVY): -0.804
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