Coding

Part:BBa_K1796101

Designed by: Tang Yulong   Group: iGEM15_SCU_China   (2015-09-17)

CODH chaperone

This sequence codes a HSP, which is helping CODH to fold in right way. If you need to produce CODH and want it to have functions, this protein is necessary.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 598
    Illegal SpeI site found at 118
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 598
    Illegal SpeI site found at 118
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 598
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 598
    Illegal SpeI site found at 118
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 598
    Illegal SpeI site found at 118
    Illegal NgoMIV site found at 654
  • 1000
    COMPATIBLE WITH RFC[1000]


Parameter of Protein

Number of amino acids: 249

Molecular weight: 26876.2

Theoretical pI: 5.65

Amino acid composition: 

Ala (A) 20 8.0%

Arg (R) 12 4.8%

Asn (N) 9 3.6%

Asp (D) 11 4.4%

Cys (C) 2 0.8%

Gln (Q) 9 3.6%

Glu (E) 17 6.8%

Gly (G) 26 10.4%

His (H) 4 1.6%

Ile (I) 25 10.0%

Leu (L) 29 11.6%

Lys (K) 12 4.8%

Met (M) 7 2.8%

Phe (F) 9 3.6%

Pro (P) 9 3.6%

Ser (S) 11 4.4%

Thr (T) 18 7.2%

Trp (W) 0 0.0%

Tyr (Y) 4 1.6%

Val (V) 15 6.0%

Pyl (O) 0 0.0%

Sec (U) 0 0.0%

(B) 0 0.0%

(Z) 0 0.0%

(X) 0 0.0%

Total number of negatively charged residues (Asp + Glu): 28

Total number of positively charged residues (Arg + Lys): 24

Atomic composition:Carbon C 1197

Hydrogen H 1959

Nitrogen N 323

Oxygen O 357

Sulfur S 9

Formula: C1197H1959N323O357S9Total number of atoms: 3845

Extinction coefficients:This protein does not contain any Trp residues. Experience shows that

this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.

Ext. coefficient 6085

Abs 0.1% (=1 g/l) 0.226, assuming all pairs of Cys residues form cystines

Ext. coefficient 5960

Abs 0.1% (=1 g/l) 0.222, assuming all Cys residues are reduced

Estimated half-life:The N-terminal of the sequence considered is V (Val).

The estimated half-life is: 100 hours (mammalian reticulocytes, in vitro).

>20 hours (yeast, in vivo).

>10 hours (Escherichia coli, in vivo).

Instability index:The instability index (II) is computed to be 27.68

This classifies the protein as stable.

Aliphatic index: 110.08

Grand average of hydropathicity (GRAVY): 0.157

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Parameters
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