Coding
Part:BBa_K1796011:Design
Designed by: Nannan Xie Group: iGEM15_SCU_China (2015-09-17)
nifN promoted from Paenibacillus sp. WLY78
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 319
Illegal PstI site found at 890
Illegal PstI site found at 961 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 319
Illegal PstI site found at 890
Illegal PstI site found at 961 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 319
Illegal PstI site found at 890
Illegal PstI site found at 961 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 319
Illegal PstI site found at 890
Illegal PstI site found at 961
Illegal NgoMIV site found at 648
Illegal AgeI site found at 25
Illegal AgeI site found at 147
Illegal AgeI site found at 766
Illegal AgeI site found at 987 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves(done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis, but unfortunately, striction enzyme cut sites were involved(three PstI) after they promoted it again. But the part can be assembled by gibson assembly,that is what we did.
Source
NifN is taken from genomic sequence of Paenibacillus sp.WLY78, we apply promotions on the sequences and synthesised it.