Coding

Part:BBa_K1796009:Design

Designed by: Nannan Xie   Group: iGEM15_SCU_China   (2015-09-17)


nifK promoted from Paenibacillus sp. WLY78


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 342
    Illegal AgeI site found at 715
    Illegal AgeI site found at 1182
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We abtained the sequences from genomes of Paenibacillus sp. WLY78. First, we promoted it ourselves（done by Nan Wang and Nannan Xie).Then we sent the sequences to synthesis,the part can be assembled by gibson assembly,that is what we did.


Source

NifK(BBa_K1796009) is taken from genomic sequence of Paenibacillus sp.WLY78, we apply promotions on the sequences and synthesised it.

References