Coding

Part:BBa_K1796006:Design

Designed by: Nannan Xie   Group: iGEM15_SCU_China   (2015-09-15)

Dinitrogenase reductase(nifH)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We put it in a pET-28b plasmid to express it. The part contains a sequence of 21bp possible RBS region.

Source

NifH(BBa_K1796006) is taken from genomic sequence of Paenibacillus sp.WLY78, we apply promotions on the sequences and synthesised it.

References

[1]Wang L, Liu Z, Zhao D, Liu X, et al.(2013) A Minimal Nitrogen Fixation Gene Cluster from Paenibacillus sp. WLY78 Enables Expression of Active Nitrogenase in Escherichia coli, PLoS Genet 9(10):e1003865.