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Part:BBa_K1791004:Design

Designed by: Graeme Glaister   Group: iGEM15_Lethbridge   (2015-09-23)


T7 promoter fused with a theophylline inducible ribozyme (aptazyme)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

by changing the T7 promoter sequence (BBa_I719005) slightly after the GGG trinulceotide we get an increase in the potential for generating RNA with our composite parts BBa_K1791001 and BBa_K1791002.


Source

Win, M.N., Smolke C.D. 2007 and BBa_I719005


References