Intermediate

Part:BBa_K179004:Design

Designed by: Hiroyuki Yamazaki   Group: iGEM09_Todai-Tokyo   (2009-10-18)


Yeast Gal1 promoter + LDLR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1273
    Illegal PstI site found at 617
    Illegal PstI site found at 1644
    Illegal PstI site found at 1908
    Illegal PstI site found at 2705
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1273
    Illegal PstI site found at 617
    Illegal PstI site found at 1644
    Illegal PstI site found at 1908
    Illegal PstI site found at 2705
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1273
    Illegal BglII site found at 2259
    Illegal BamHI site found at 1632
    Illegal BamHI site found at 2003
    Illegal BamHI site found at 2127
    Illegal XhoI site found at 3099
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1273
    Illegal PstI site found at 617
    Illegal PstI site found at 1644
    Illegal PstI site found at 1908
    Illegal PstI site found at 2705
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1273
    Illegal PstI site found at 617
    Illegal PstI site found at 1644
    Illegal PstI site found at 1908
    Illegal PstI site found at 2705
    Illegal NgoMIV site found at 1310
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

LDLR is difficult to amplify with PCR


Source

Gal1 promoter is BBa_J63006, while LDLR from human genome sequence.

References