Coding

Part:BBa_K179003:Design

Designed by: Koji Tanaka   Group: iGEM09_Todai-Tokyo   (2009-10-18)


Low density lipoprotein receptor


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 718
    Illegal PstI site found at 62
    Illegal PstI site found at 1089
    Illegal PstI site found at 1353
    Illegal PstI site found at 2150
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 718
    Illegal PstI site found at 62
    Illegal PstI site found at 1089
    Illegal PstI site found at 1353
    Illegal PstI site found at 2150
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 718
    Illegal BglII site found at 1704
    Illegal BamHI site found at 1077
    Illegal BamHI site found at 1448
    Illegal BamHI site found at 1572
    Illegal XhoI site found at 2544
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 718
    Illegal PstI site found at 62
    Illegal PstI site found at 1089
    Illegal PstI site found at 1353
    Illegal PstI site found at 2150
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 718
    Illegal PstI site found at 62
    Illegal PstI site found at 1089
    Illegal PstI site found at 1353
    Illegal PstI site found at 2150
    Illegal NgoMIV site found at 755
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This has many restriction enzyme cutting sites; 1 EcoRI cutting site and 4 PstI cutting site.


Source

This comes from human LDLR cDNA

References