Coding

Part:BBa_K1783005:Design

Designed by: Kara Huie, Juhye Kang, Iowis Zhu   Group: iGEM15_UMaryland   (2015-08-14)


Epsilon Hydroxylase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 39
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 346
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This BioBrick was created by DNA synthesis through IDT. The gBlock of this part was cloned into a linearized pSB1C3 backbone with Gibson assembly. Codon optimized using IDT Codon Optimizer.

Source

Genome of Arabidopsis thaliana.

References