Protein_Domain

Part:BBa_K1781002:Design

Designed by: Marvin Prein   Group: iGEM15_TU_Dresden   (2015-09-09)


ZHER2 - affibody with affinity to cytoplasmic domain of HER2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 220
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As it is only 58 amino acids long, the small size of the sequence can be problematic towards cloning and recombineering procedures


Source

The sequence was received from the scientific paper by Charles Eigenbrot in 2010, who engineered ZHER2 to have a better affinity towards HER2.

References