DNA

Part:BBa_K1778006:Design

Designed by: Lehua Jia   Group: iGEM15_Scut-Champion-Park   (2015-09-11)


Kana-His is a selective marker gene in experiment.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 446
    Illegal PstI site found at 3038
    Illegal PstI site found at 4279
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 207
    Illegal NheI site found at 1154
    Illegal PstI site found at 3038
    Illegal PstI site found at 4279
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 4123
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 446
    Illegal PstI site found at 3038
    Illegal PstI site found at 4279
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 446
    Illegal PstI site found at 3038
    Illegal PstI site found at 4279
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2494
    Illegal BsaI site found at 4595


Design Notes

Because the plasmid pPIC9K has been widely used, we just need to get the segment using PCR.


Source

Kana-His is from plasmid pPIC9K.

References