Generator

Part:BBa_K177043

Designed by: Marcin Ziemniak   Group: iGEM09_Warsaw   (2009-09-18)

Tetracycline repressor under RBS.3 (medium)

Coding region for the TetR protein connected to medium RBS. CDS of TetR is modified with an LVA tail for rapid degradation of the protein. TetR binds to the pTet regulator (BBa_R0040). Anhydrotetracycline binds to TetR and inhibits its operation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None