Inverter

Part:BBa_K1768003:Design

Designed by: Gert Pietersen   Group: iGEM15_Pretoria_UP   (2015-09-14)


Recombinase invertible constitutive promoter 2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 115
    Illegal NheI site found at 138
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Lox71 is reversed relative to Lox66. Spacer sequence from non-coding pUC19 are added between Lox sites.


Source

Lox66: BBa_I718016 Lox71: BBa_J23100 Constitutive promoter: BBa_J23100

References