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Part:BBa_K1766009:Design

Designed by: Pontus Höjer   Group: iGEM15_Stockholm   (2015-09-13)


BAR construct 1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 387
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 236
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The bacterial antigen receptor (BAR), construct 1, was created by replacing part of the periplasmic domain of EnvZ. Structure prediction of the periplasmic domain of EnvZ revealed a possible PAS domain with a Beta-sheet->Coil->Alpha-helix->Coil structure. This structure was replaced with the Z:HER2:342 affibody with documented affinity towards the human epidermal growth factor receptor (HER2).

Source

Part created through overlap PCR using EnvZ_V5 BBa_K1766008 and Z:HER2:342.

References