Part:BBa_K1763439:Design

MaSp1/2[2:1]-12(T7)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1302
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We chose to express BBa_K1763438 under control of the T7-RBS system rather than the Lac operon due to our access to well characterized BL21(DE3) expression systems.

This construct was assembled by subcloning BBa_K1763438 into BBa_K525998.

This part was sequence verified using registry standard VF2 and VR primers.

Source

BBa_K1763438 was created by our team. BBa_K525998 was obtained through the 2015 Registry Distribution.

References

Briggs A., Rios X., Chari R., Luhan Y., Zhang F., Mali P., and Church G. Iterative capped assembly: rapid and scalable synthesis of repeat-module DNA such as TAL effectors from individual monomers. Nucleic Acids Research. 2012;40(15): e117

Hinman, M.B., Lewis, R. Isolation of a clone encoding a second dragline silk fibroin. Nephila clavipes dragline silk is a two-protein fiber. J. Biol. Chem. 1992;267: 19320–19324.