Device
Part:BBa_K176249
Designed by: Chao Li, Hao Jiang Group: iGEM09_USTC (2009-10-15)
Low input score automatic selector w/o lethal device(with reporter): PoPS->mRFP->CI-LVA+pCI->K176240
PoPS->mRFP->cI-LVA+pCI->luxI-LVA->tetR-LVA + pCon 0.15->luxI-LVA + pCon 0.70->luxR + pLux/Tet->PoPS
Output PoPS = (Cell Density * (pCon 0.15 + Population Average (1 / (1 + Input PoPS))) + Input AHL) * Input PoPS
If output is a lethal device, cells with relative low input score in the population will be selected, cells with relative high input score will die.
The first luxI-LVA is used to calculate average score in the population.
mRFP is input score reporter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 3251
Illegal NheI site found at 3274
Illegal NheI site found at 4100
Illegal NheI site found at 4123 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2382
Illegal BglII site found at 3942 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 573
Illegal AgeI site found at 685 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 5078
[edit]
Categories
Parameters
n/a | Low input score automatic selector w/o lethal device(with reporter): PoPS->mRFP->CI-LVA+pCI->K176240 |