Device

Part:BBa_K176249

Designed by: Chao Li, Hao Jiang   Group: iGEM09_USTC   (2009-10-15)

Low input score automatic selector w/o lethal device(with reporter): PoPS->mRFP->CI-LVA+pCI->K176240

PoPS->mRFP->cI-LVA+pCI->luxI-LVA->tetR-LVA + pCon 0.15->luxI-LVA + pCon 0.70->luxR + pLux/Tet->PoPS

Output PoPS = (Cell Density * (pCon 0.15 + Population Average (1 / (1 + Input PoPS))) + Input AHL) * Input PoPS

If output is a lethal device, cells with relative low input score in the population will be selected, cells with relative high input score will die.

The first luxI-LVA is used to calculate average score in the population.

mRFP is input score reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3251
    Illegal NheI site found at 3274
    Illegal NheI site found at 4100
    Illegal NheI site found at 4123
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2382
    Illegal BglII site found at 3942
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 573
    Illegal AgeI site found at 685
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5078


[edit]
Categories
Parameters
n/aLow input score automatic selector w/o lethal device(with reporter): PoPS->mRFP->CI-LVA+pCI->K176240