Device
Part:BBa_K176247
Designed by: Chao Li, Hao Jiang Group: iGEM09_USTC (2009-10-15)
High input score automatic selector without lethal device (with reporter): PoPS->mRFP->K176240->PoPS
PoPS->mRFP->luxI-LVA->tetR-LVA + pCon 0.15->luxI-LVA + pCon 0.70->luxR + pLux/Tet->PoPS
Output PoPS = (Cell Density * (pCon 0.15 + Population Average Input PoPS) + Input AHL) / (1 + Input PoPS)
If output is a lethal device, cells with relative high input score in the population will be selected, cells with relative low input score will die.
The first luxI-LVA is used to calculate average score in the population.
mRFP is input score reporter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2256
Illegal NheI site found at 2279
Illegal NheI site found at 3105
Illegal NheI site found at 3128 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1387
Illegal BglII site found at 2947 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 573
Illegal AgeI site found at 685 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 4083
[edit]
Categories
Parameters
n/a | High input score automatic selector without lethal device (with reporter): PoPS->mRFP->K176240->PoPS |