Device

Part:BBa_K176232:Design

Designed by: Danqian Liu, Zongxiao He, Hao Jiang   Group: iGEM09_USTC   (2009-10-13)


For characterization of K176230: VR7+pCon 0.36->K176230


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 35
    Illegal NheI site found at 58
    Illegal NheI site found at 2341
    Illegal NheI site found at 2364
    Illegal NheI site found at 3190
    Illegal NheI site found at 3213
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1472
    Illegal BglII site found at 3032
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4657
    Illegal BsaI.rc site found at 733
    Illegal BsaI.rc site found at 4168


Design Notes

VR7+pCon 0.36 as input for characterization of K176230.

VR7+pCon 0.36->GFP->luxI-LVA->tetR-LVA + pCon 0.15->luxI-LVA + pCon 0.70->luxR + pLux/Tet->lacZalpha-ccdB(weak RBS)


Source

K176102 K176230

References