Device

Part:BBa_K176226

Designed by: Danqian Liu, Zongxiao He, Hao Jiang   Group: iGEM09_USTC   (2009-10-13)

Repressible cell density controller: PoPS->tetR-LVA->K176096

PoPS->tetR-LVA+pCon 0.15->luxI-LVA+pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)

Cell Death = ((Cell Density * pCon 0.15) + Input AHL) / (1 + Input PoPS)

High Input PoPS -> High Steady State Cell Density

High Input AHL -> Low Steady State Cell Density

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 855
    Illegal NheI site found at 878
    Illegal NheI site found at 1704
    Illegal NheI site found at 1727
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1546
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3171
    Illegal BsaI.rc site found at 2682


Model

To see the details of the model, click [http://2009.igem.org/Team:USTC/Modeling/Model-9 here] please.



[edit]
Categories
Parameters
n/aRepressible cell density controller: PoPS->tetR-LVA->K176096