Device

Part:BBa_K176225:Design

Designed by: Danqian Liu, Zongxiao He, Hao Jiang   Group: iGEM09_USTC   (2009-10-10)


PoPS->AID(week RBS)->mRFP->CI Inverter (cI-LVA+pCI)->luxI-LVA->PoPS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3010
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1201
    Illegal AgeI site found at 1313
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 96
    Illegal SapI site found at 197


Design Notes

Input: PoPS

Output1: Mutation Rate

Output2: mRFP

Output3: LuxI-LVA

Output4: PoPS

Design:

Input PoPS generates AID (weak RBS), Output2 mRFP and CI-LVA, AID increases Output1 Mutation Rate.

pCI generates Output3 LuxI-LVA and Output3 PoPS, repressed by CI-LVA.


Source

K176191 K176224

References