Signalling

Part:BBa_K176170:Design

Designed by: Danqian Liu, Zongxiao He, Hao Jiang   Group: iGEM09_USTC   (2009-10-06)

TetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA(weak RBS)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1474
    Illegal BsaI.rc site found at 985


Design Notes

Input: AHL

Output: cell death

Design:

pCon 0.70(J23101) generates LuxR

pLux/Tet(K176000) generates lacZalpha-ccdB-LVA (weak RBS) to kill the cell, activated by LuxR and AHL, repressed by TetR and no aTc


Source

K176028 K176166

References