Generator

Part:BBa_K1758370:Design

Designed by: Team Bielefeld-CeBiTec 2015   Group: iGEM15_Bielefeld-CeBiTec   (2015-08-27)


BlcR, repressor of blcABC operon, with promoter and RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 812
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 520
    Illegal NgoMIV site found at 761
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BlcR coding sequence was cloned into pSB1C3 with Gibson primers


Source

This sequence comes from megaplasmid pATC58 from Agrobacterium tumefaciens strain C58.1

References