Generator
Part:BBa_K1741006:Design
Designed by: Marta Żardecka Group: iGEM15_UAM_Poznan (2015-09-17)
sfGFP under short rhamnose promoter (very weak)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 148
Design Notes
We removed both CPR sites.
Source
All promoter fragments derive from Escherichia coli K12, strain DH5 alpha, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section.