Generator

Part:BBa_K1741002:Design

Designed by: Marta Żardecka   Group: iGEM15_UAM_Poznan   (2015-09-17)


sfGFP under a short arabinose promoter s2 without O1 and O2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 54
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 170


Design Notes

We removed O1 and O2 regions from the promoter.


Source

All promoter fragments derive from Escherichia coli K12, strain DH5 alpha, some elements are synthetic. Chromosomal DNA of E. coli was isolated as described in the methods section.

References

New shorter versions of arabinose induced promoters, all originating from E. coli genome were compared to the biobrick BBa_K1481002 (https://parts.igem.org/Part:BBa_K1481002), provided last year by Poznan_Bioinf team.