Coding
Part:BBa_K174019:Design
Designed by: The Newcastle 2009 iGEM team Group: iGEM09_Newcastle (2009-10-21)
SmtA metallothionein protein as a fusion partner
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 118
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence does not start with ATG. So if anyone wants to use it without any fusion, then ATG start codon should be added to the beginning of the sequence.
Source
E. coli
References
- Cretì, P., F. Trinchella, et al. "Heavy metal bioaccumulation and metallothionein content in tissues of the sea bream Sparus aurata from three different fish farming systems." Environmental Monitoring and Assessment.
- Morby, A. P., J. S. Turner, et al. (1993). SmtB is a metal-dependent repressor of the cyanobacterial metallothionein gene smtA: identification of a Zn inhibited DNA-protein complex. 21: 921-925.
- Waldron, K. J. and N. J. Robinson (2009). "How do bacterial cells ensure that metalloproteins get the correct metal?" Nat Rev Micro 7(1): 25-35.