Regulatory

Part:BBa_K174015:Design

Designed by: The Newcastle 2009 iGEM team   Group: iGEM09_Newcastle   (2009-10-14)

Cadmium Sensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 200
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We wanted to sense cadmium accurately and used a combinatorial approach to achieve our goal.


Source

cadA promoter which has the binding site for CzrA protein is combined with ArsR binding site to create this AND gate cadmium sensor.

References