Coding

Part:BBa_K1732023:Design

Designed by: Donna Lee   Group: iGEM15_Carnegie_Mellon   (2015-09-18)


OFPCO-His


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 570
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

His tag for easier protein purification and sequence was codon optimized for E. coli.


Source

BBa_K1732011

References