DNA

Part:BBa_K1732005:Design

Designed by: Donna Lee   Group: iGEM15_Carnegie_Mellon   (2015-09-09)

J23100-B0034-Renilla-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 832
    Illegal XhoI site found at 641
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 265
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pSB1C3 backbone (part:pSB1C3) with J23100 promoter (BBa_J23100) and RBS (BBa_B0034), Renilla (BBa_52008), and B0015 terminator (BBa_B0015).

Sequence is codon optimized for E. coli which is why the Renilla luciferase sequence differs from the original BBa_52008 part)


Source

Sequence designed by Cheryl Telmer via IDT. Codon optimized Renilla Luciferase to allow optimal productions of Rluc.

References